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KMID : 0869420160140010001
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Journal of Jeahan Oriental Medical Academy
2016 Volume.14 No. 1 p.1 ~ p.9
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Hepatoprotective effect of the CEE(ethanol extract of Citrus unshiu Markovich epicarp) depending on the storage period
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Noh Gyu-Pyo
Cho Il-Je
Park Chung-A
Kim Sang-Chan
Byun Sung-Hui
Park Sook-Ja
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Abstract
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Objectives : This study aimed to investigate the change of hepatoprotective activity depending on the storage period of CEE (ethanol extract of Citrus unshiu Markovich epicarp).
Methods : Citrus unshiu Markovich epicarp was washed, sliced and dried for 7 days at room temperature, and it was considered as zero month time. Citrus unshiu Markovich epicarp was further stored for 2, 4 and 6 months in the dry oven, which was set to 30℃. Hepatotoxicity was induced by exposing HepG2 cells to 100 μM cadmium for 3 hours. Cell viability was determined by MTT assay.
Results : CEE showed hepatoprotective effect better than water extract of Citrus unshiu Markovich epicarp in cadmium-treated HepG2 cells. Thus, we performed the cytoprotective efficacy experiment using CEE. Significant hepatoprotective activity was found in the all concentrations of CEE used in the experiment (10, 30, 100 ㎍/㎖). Depending on the prolonged storage period, CEE showed slightly weak hepatoprotective effect on average. However, there was no statistical significance in the change of hepatoprotective effect.
Conclusions : CEE showed dose-dependent hepatoprotective activity, and the significant difference in activity over storage time was not found. In conclusion, CEE can be used without change in activity, even if stored for 6 months at room temperature. In addition, the storage time of more than 6 months is recommended, to determine that the activity of Citrus unshiu Markovich epicarp is better depending on the storage period.
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KEYWORD
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Ethanol extracts of Citrus unshiu Markovich epicarp (CEE), Cadmium, Hepatoprotective, Storage period
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